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flow cytometry analysis  (Sartorius AG)


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    Structured Review

    Sartorius AG flow cytometry analysis
    Flow Cytometry Analysis, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 97/100, based on 476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+analysis/Flow+Cytometry+system/pmc13109719-113-9-12
    Average 97 stars, based on 476 article reviews
    flow cytometry analysis - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Staining:

    Article Title: The VLDLR entry receptor is required for the pathogenesis of multiple encephalitic alphaviruses
    Article Snippet: Cells were washed and then incubated with Alexa Fluor 647-conjugated goat anti-human or anti-mouse IgG (1:2000 dilution in HBSS-BN; Thermo Fisher) for 15 min at 4°C. .. Cells were washed and resuspended in HBSS-BN containing 4′4,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) to stain dead cells and subjected to flow cytometry analysis using an iQue3 flow cytometer (Sartorius). .. A pCAGGS vector encoding WEEV McMillan structural genes was modified to remove a putative nuclear localization signal in the capsid (K67N, K69N, K82N, K84N, K91N) by Gibson assembly of GeneBlocks (IDT).

    Article Title: Compositions and methods for treating age-related macular degeneration
    Article Snippet: .. Propidium Iodide Staining and Flow Cytometry Analysis Following rescue studies as described above, RPE cells (3×105) at passage 3 were harvested with 0.25% trypsin/EDTA (Biological Industries). .. Cells were collected by centrifugation at 500 g for 5 min, washed twice with phosphate-buffered saline (PBS), and re-suspended in 400 μl of PBS to which 1 μl of propidium iodide (Sigma) 1 mg/ml was added immediately before flow cytometry measurements.

    Article Title: Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica.
    Article Snippet: .. Cell staining and flow cytometry Prior to flow cytometry analysis, 2 mL of culture broth was washed with 5 mL of phosphate buffer (8 g/L NaCl, 0.2 g/L KCl, 1.44 g/L Na2HPO4, 0.24 g/L KH2PO4, pH 7.2) via vacuum filtration with a filter of a pore size of 0.2 μm (Sartorius Stedim, Göttingen, Germany) directly after sampling, and resuspended in 10 mL of buffer. .. Samples were measured directly using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) at an extinction wavelength λex = 488 nm from an argon-ion laser source at 25 mW and detection at an emission wavelength λem = 580 nm.

    Article Title: Structural basis for plasticity in receptor engagement by an encephalitic alphavirus.
    Article Snippet: .. Cells were washed and then incubated with Alexa Fluor 647-conjugated goat anti-human or anti-mouse IgG (1 mg/mL) for 15min at 4 C. Cells were washed and resuspended in HBSSBN or FACS buffer containing 4’4,6-diamidino-2-phenylindole (DAPI, 1 mg/mL) to stain dead cells and subjected to flow cytometry analysis using an iQue3 flow cytometer (Sartorius). .. Ectopic expression and stable cell line generation K562 cells stably expressing N-terminal Flag-tagged versions of mouse MXRA8, chicken MXRA8, and VLDLR have been previously described.6,8,16 A codon-optimized H. sapiens (human) PCDH10 (NM_032961.3) or P. montanus (sparrow) PCDH10 (XM_039733439.1) with the endogenous signal sequence and internal Flag-tag inserted between residues S696 and G697 (H. sapiens PCDH10) was synthesized and cloned into the pSFFV-IRES-puromycin lentiviral transfer vector by Twist Biosciences.

    Flow Cytometry:

    Article Title: The VLDLR entry receptor is required for the pathogenesis of multiple encephalitic alphaviruses
    Article Snippet: Cells were washed and then incubated with Alexa Fluor 647-conjugated goat anti-human or anti-mouse IgG (1:2000 dilution in HBSS-BN; Thermo Fisher) for 15 min at 4°C. .. Cells were washed and resuspended in HBSS-BN containing 4′4,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) to stain dead cells and subjected to flow cytometry analysis using an iQue3 flow cytometer (Sartorius). .. A pCAGGS vector encoding WEEV McMillan structural genes was modified to remove a putative nuclear localization signal in the capsid (K67N, K69N, K82N, K84N, K91N) by Gibson assembly of GeneBlocks (IDT).

    Article Title: Compositions and methods for treating age-related macular degeneration
    Article Snippet: .. Propidium Iodide Staining and Flow Cytometry Analysis Following rescue studies as described above, RPE cells (3×105) at passage 3 were harvested with 0.25% trypsin/EDTA (Biological Industries). .. Cells were collected by centrifugation at 500 g for 5 min, washed twice with phosphate-buffered saline (PBS), and re-suspended in 400 μl of PBS to which 1 μl of propidium iodide (Sigma) 1 mg/ml was added immediately before flow cytometry measurements.

    Article Title: Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells
    Article Snippet: .. T cell proliferation was assessed by transferring 50 μL of the cell samples to a PBS-DAPI plate for flow cytometry analysis, and functional T cell persistence was monitored by live cell imaging of GFP+ target cells over time using an IncuCyte device (Sartorius). .. The following steps were carried out in an amplicon-free pre-PCR area; 500 ng of genomic DNA was amplified using NEBNext Ultra II Q5 HiFi polymerase (New England Biolabs) with primers containing stubbers for downstream indexing (TRAC_ONT_F: 5′-TTTCTGTTGGTGCTGATATTGCTTCAATCACTGCTGTGTCCCT-3’; TRAC_ONT_R: 5′-ACTTGCCTGTCGCTCTATCTTCTCCCACCCCAGACCTCCTAGTT-3′).

    Article Title: Enhanced Bioactivity of Puerariae Radix‐ Hovenia Seed Extracts Through Lactiplantibacillus plantarum and Lacticaseibacillus paracasei Co‐Fermentation: Impact on Alcoholic Liver Injury and Macrophage Polarization
    Article Snippet: .. Ultimately, a flow cytometer was employed to conduct the flow cytometry analysis (Sartorius, Argentina, German) following standard settings (excitation light 488and 642 nm) (Njoroge et al. ). ..

    Article Title: Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica.
    Article Snippet: .. Cell staining and flow cytometry Prior to flow cytometry analysis, 2 mL of culture broth was washed with 5 mL of phosphate buffer (8 g/L NaCl, 0.2 g/L KCl, 1.44 g/L Na2HPO4, 0.24 g/L KH2PO4, pH 7.2) via vacuum filtration with a filter of a pore size of 0.2 μm (Sartorius Stedim, Göttingen, Germany) directly after sampling, and resuspended in 10 mL of buffer. .. Samples were measured directly using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) at an extinction wavelength λex = 488 nm from an argon-ion laser source at 25 mW and detection at an emission wavelength λem = 580 nm.

    Article Title: Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno-associated virus productivity across different HEK293 cell lines.
    Article Snippet: Correspondence Niklas Krämer, Institute of Cell Culture Technology, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany.. Email: niklas.kraemer@sartorius.com Abstract Recombinant adeno-associated viruses (rAAV) are one of the most popular gene therapy vectors.. To date, low-product yields are limiting a broader clinical application.

    Article Title: Sequencing of the cDNA variable region of a lumpy skin disease virus ORF123 monoclonal antibody and its cross-neutralizing activity analyses against Capripoxvirus members.
    Article Snippet: Monoclonal antibody sequencing is crucial for enhancing our understanding of the relationship between antibody neutralization and broad-spectrum binding.. Our previous study systematically described a B-cell monoclonal antibody (mAb) derived from lumpy skin disease virus (LSDV) ORF123, which exhibits cross-reactivity with goatpoxvirus (GTPV) and sheeppoxvirus (SPPV).. Here, the LSDV ORF123 mAb was sequenced for the first time using the HybSeq HTTM next-generation sequencing technique.

    Article Title: Structural basis for plasticity in receptor engagement by an encephalitic alphavirus.
    Article Snippet: .. Cells were washed and then incubated with Alexa Fluor 647-conjugated goat anti-human or anti-mouse IgG (1 mg/mL) for 15min at 4 C. Cells were washed and resuspended in HBSSBN or FACS buffer containing 4’4,6-diamidino-2-phenylindole (DAPI, 1 mg/mL) to stain dead cells and subjected to flow cytometry analysis using an iQue3 flow cytometer (Sartorius). .. Ectopic expression and stable cell line generation K562 cells stably expressing N-terminal Flag-tagged versions of mouse MXRA8, chicken MXRA8, and VLDLR have been previously described.6,8,16 A codon-optimized H. sapiens (human) PCDH10 (NM_032961.3) or P. montanus (sparrow) PCDH10 (XM_039733439.1) with the endogenous signal sequence and internal Flag-tag inserted between residues S696 and G697 (H. sapiens PCDH10) was synthesized and cloned into the pSFFV-IRES-puromycin lentiviral transfer vector by Twist Biosciences.

    Transferring:

    Article Title: Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells
    Article Snippet: .. T cell proliferation was assessed by transferring 50 μL of the cell samples to a PBS-DAPI plate for flow cytometry analysis, and functional T cell persistence was monitored by live cell imaging of GFP+ target cells over time using an IncuCyte device (Sartorius). .. The following steps were carried out in an amplicon-free pre-PCR area; 500 ng of genomic DNA was amplified using NEBNext Ultra II Q5 HiFi polymerase (New England Biolabs) with primers containing stubbers for downstream indexing (TRAC_ONT_F: 5′-TTTCTGTTGGTGCTGATATTGCTTCAATCACTGCTGTGTCCCT-3’; TRAC_ONT_R: 5′-ACTTGCCTGTCGCTCTATCTTCTCCCACCCCAGACCTCCTAGTT-3′).

    Functional Assay:

    Article Title: Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells
    Article Snippet: .. T cell proliferation was assessed by transferring 50 μL of the cell samples to a PBS-DAPI plate for flow cytometry analysis, and functional T cell persistence was monitored by live cell imaging of GFP+ target cells over time using an IncuCyte device (Sartorius). .. The following steps were carried out in an amplicon-free pre-PCR area; 500 ng of genomic DNA was amplified using NEBNext Ultra II Q5 HiFi polymerase (New England Biolabs) with primers containing stubbers for downstream indexing (TRAC_ONT_F: 5′-TTTCTGTTGGTGCTGATATTGCTTCAATCACTGCTGTGTCCCT-3’; TRAC_ONT_R: 5′-ACTTGCCTGTCGCTCTATCTTCTCCCACCCCAGACCTCCTAGTT-3′).

    Live Cell Imaging:

    Article Title: Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells
    Article Snippet: .. T cell proliferation was assessed by transferring 50 μL of the cell samples to a PBS-DAPI plate for flow cytometry analysis, and functional T cell persistence was monitored by live cell imaging of GFP+ target cells over time using an IncuCyte device (Sartorius). .. The following steps were carried out in an amplicon-free pre-PCR area; 500 ng of genomic DNA was amplified using NEBNext Ultra II Q5 HiFi polymerase (New England Biolabs) with primers containing stubbers for downstream indexing (TRAC_ONT_F: 5′-TTTCTGTTGGTGCTGATATTGCTTCAATCACTGCTGTGTCCCT-3’; TRAC_ONT_R: 5′-ACTTGCCTGTCGCTCTATCTTCTCCCACCCCAGACCTCCTAGTT-3′).

    Suction Filtration:

    Article Title: Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica.
    Article Snippet: .. Cell staining and flow cytometry Prior to flow cytometry analysis, 2 mL of culture broth was washed with 5 mL of phosphate buffer (8 g/L NaCl, 0.2 g/L KCl, 1.44 g/L Na2HPO4, 0.24 g/L KH2PO4, pH 7.2) via vacuum filtration with a filter of a pore size of 0.2 μm (Sartorius Stedim, Göttingen, Germany) directly after sampling, and resuspended in 10 mL of buffer. .. Samples were measured directly using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) at an extinction wavelength λex = 488 nm from an argon-ion laser source at 25 mW and detection at an emission wavelength λem = 580 nm.

    Pore Size:

    Article Title: Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica.
    Article Snippet: .. Cell staining and flow cytometry Prior to flow cytometry analysis, 2 mL of culture broth was washed with 5 mL of phosphate buffer (8 g/L NaCl, 0.2 g/L KCl, 1.44 g/L Na2HPO4, 0.24 g/L KH2PO4, pH 7.2) via vacuum filtration with a filter of a pore size of 0.2 μm (Sartorius Stedim, Göttingen, Germany) directly after sampling, and resuspended in 10 mL of buffer. .. Samples were measured directly using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) at an extinction wavelength λex = 488 nm from an argon-ion laser source at 25 mW and detection at an emission wavelength λem = 580 nm.

    Sampling:

    Article Title: Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica.
    Article Snippet: .. Cell staining and flow cytometry Prior to flow cytometry analysis, 2 mL of culture broth was washed with 5 mL of phosphate buffer (8 g/L NaCl, 0.2 g/L KCl, 1.44 g/L Na2HPO4, 0.24 g/L KH2PO4, pH 7.2) via vacuum filtration with a filter of a pore size of 0.2 μm (Sartorius Stedim, Göttingen, Germany) directly after sampling, and resuspended in 10 mL of buffer. .. Samples were measured directly using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) at an extinction wavelength λex = 488 nm from an argon-ion laser source at 25 mW and detection at an emission wavelength λem = 580 nm.

    Incubation:

    Article Title: Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno-associated virus productivity across different HEK293 cell lines.
    Article Snippet: Correspondence Niklas Krämer, Institute of Cell Culture Technology, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany.. Email: niklas.kraemer@sartorius.com Abstract Recombinant adeno-associated viruses (rAAV) are one of the most popular gene therapy vectors.. To date, low-product yields are limiting a broader clinical application.

    Article Title: Structural basis for plasticity in receptor engagement by an encephalitic alphavirus.
    Article Snippet: .. Cells were washed and then incubated with Alexa Fluor 647-conjugated goat anti-human or anti-mouse IgG (1 mg/mL) for 15min at 4 C. Cells were washed and resuspended in HBSSBN or FACS buffer containing 4’4,6-diamidino-2-phenylindole (DAPI, 1 mg/mL) to stain dead cells and subjected to flow cytometry analysis using an iQue3 flow cytometer (Sartorius). .. Ectopic expression and stable cell line generation K562 cells stably expressing N-terminal Flag-tagged versions of mouse MXRA8, chicken MXRA8, and VLDLR have been previously described.6,8,16 A codon-optimized H. sapiens (human) PCDH10 (NM_032961.3) or P. montanus (sparrow) PCDH10 (XM_039733439.1) with the endogenous signal sequence and internal Flag-tag inserted between residues S696 and G697 (H. sapiens PCDH10) was synthesized and cloned into the pSFFV-IRES-puromycin lentiviral transfer vector by Twist Biosciences.

    Expressing:

    Article Title: Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno-associated virus productivity across different HEK293 cell lines.
    Article Snippet: Correspondence Niklas Krämer, Institute of Cell Culture Technology, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany.. Email: niklas.kraemer@sartorius.com Abstract Recombinant adeno-associated viruses (rAAV) are one of the most popular gene therapy vectors.. To date, low-product yields are limiting a broader clinical application.

    Transfection:

    Article Title: Sequencing of the cDNA variable region of a lumpy skin disease virus ORF123 monoclonal antibody and its cross-neutralizing activity analyses against Capripoxvirus members.
    Article Snippet: Monoclonal antibody sequencing is crucial for enhancing our understanding of the relationship between antibody neutralization and broad-spectrum binding.. Our previous study systematically described a B-cell monoclonal antibody (mAb) derived from lumpy skin disease virus (LSDV) ORF123, which exhibits cross-reactivity with goatpoxvirus (GTPV) and sheeppoxvirus (SPPV).. Here, the LSDV ORF123 mAb was sequenced for the first time using the HybSeq HTTM next-generation sequencing technique.

    FACS:

    Article Title: Structural basis for plasticity in receptor engagement by an encephalitic alphavirus.
    Article Snippet: .. Cells were washed and then incubated with Alexa Fluor 647-conjugated goat anti-human or anti-mouse IgG (1 mg/mL) for 15min at 4 C. Cells were washed and resuspended in HBSSBN or FACS buffer containing 4’4,6-diamidino-2-phenylindole (DAPI, 1 mg/mL) to stain dead cells and subjected to flow cytometry analysis using an iQue3 flow cytometer (Sartorius). .. Ectopic expression and stable cell line generation K562 cells stably expressing N-terminal Flag-tagged versions of mouse MXRA8, chicken MXRA8, and VLDLR have been previously described.6,8,16 A codon-optimized H. sapiens (human) PCDH10 (NM_032961.3) or P. montanus (sparrow) PCDH10 (XM_039733439.1) with the endogenous signal sequence and internal Flag-tag inserted between residues S696 and G697 (H. sapiens PCDH10) was synthesized and cloned into the pSFFV-IRES-puromycin lentiviral transfer vector by Twist Biosciences.



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